Gas Chromatographic determination of parabens after derivatization and dispersive microextraction

Authors

  • Valentyna M. Levchyk Kiev Taras Shevchenko National University
  • Marina F. Zui Kiev Taras Shevchenko National University

DOI:

https://doi.org/10.17721/fujcV3I2P72-79

Keywords:

paraben, DLLME, derivatization

Abstract

Abstract. In this study a dispersive liquid–liquid microextraction  method combines with an in situ derivatization. The proposed method is used for the extraction and pre-concentration of some preservatives including methyl paraben, ethyl paraben, propyl paraben and butyl paraben from different water matrices. The extracted compounds are monitored by gas chromatography-flame ionization detector.  Parabens are derivatizated by propionic anhydride. The effects of the extraction solvent type, extraction and acylation time, derivative agent volume, temperature, pH and the ionic strength of the solution on the extraction efficiency were investigated. 50 mkl chloroform and 500 mkl acetonitrile is a mixture of suitable extraction and dispersive solvents. The mixture is centrifuged for 3min at 4000 rpm. 20 mkl propionic anhydride is used for derivatization parabens in the concentration range of 0.05 - 1 mg  within 2 - 3 minutes. The optimum pH range is 8 - 9. The relative standard deviations (RSDs) of parabens were in the range of 2.0–10.0% (n = 3). The method was applied to the analysis of the four parabens in water. For the analysis of the spiked samples, a recovery above 100 % were obtained.

Author Biography

Valentyna M. Levchyk, Kiev Taras Shevchenko National University

chemistry department

References

Ocaña-González J, Villar-Navarro M, Ramos-Payán M, Fernández-Torres R, Bello-López M. New developments in the extraction and determination of parabens in cosmetics and environmental samples. A review. Analytica Chimica Acta 2015;858:1-15. https://doi.org/10.1016/j.aca.2014.07.002

Baranowska I, Wojciechowska I. The Determination of Preservatives in Cosmetics and Environmental Waters by HPLC. Pol. J. Environ. Study 2013;22(6):1609-94.

Scientific committee on consumer safety (SCCS). Opinion on parabens. COLIPA P82. SCCS/1348/10 1348;2011(1):38.

Zimmerman DC, Rossi HF, Keating DW. Agilent Technologies, Printed in the USA. 2013.

Saraji M, Mirmahdieh S. Single-drop microextraction followed by in-syringe derivatization and GC-MS detection for the determination of parabens in water and cosmetic products. J. Sep. Sci. 2009;32(7):988-995. https://doi.org/10.1002/jssc.200800635

Prichodko A, Šakočiūtė V, Vičkačkaitė V. Chemija 2010; 21(2):112.

Prichodko A, Janenaite E, Smitiene V, Vickackaite V. Gas chromatographic determination of parabens after in-situ derivatization and dispersive liquid-liquid microextraction . Acta Chromatographica 2012;24(4):589-601. https://doi.org/10.1556/achrom.24.2012.4.6

Downloads

Published

2015-12-15